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Projeto Externo - de Outra Unidade da USP

Dados do solicitante

Filippo Giovanni Ghiglieno

Natureza do projeto

Projeto de docente ou pesquisador
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Projeto de Pós-Doutorado

Pesquisadores ou docentes associados

Filippo Giovanni Ghiglieno
Bruno Monti Oliveira
Maiá Gomes Medeiros
Rubens Mendes Lopes

Recursos

Bolsa de Pós-Doutorado - Filippo Ghiglieno
Fapesp

Descrição do projeto

Pesquisa Aplicada em Holografia para Análise de Imagens do Plankton
Holographic Applied Research for Plankton Image Analysis (HARPIA)
01-05-2012
30-04-2015
Digital holography represents an increasingly promising approach for underwater applications. Competitive technological solutions arising from novel interdisciplinary studies are progressively paving the way for digital holography as a competitive tool for the enumeration and identification of particles and microscopic organisms in large-volume water samples. However, a challenging problem still preventing a widespread use of digital holography in fluidic applications is the massive amount of information generated by minute-range video sequences. The main goal of the HARPIA project is to develop the first software capable of processing large holographic image datasets in near-real time. At the moment no commercial product can meet these requirements. Merging the know-how of the Oceanographic Institute, University of São Paulo (LAPS/IOUSP). Worldwide computational efforts are related to reduce both the time-machine for speeding, on advanced optical components, with computer science solutions to be developed in the
HARPIA project, we will implement integrated software solutions for the identification, measurement and enumeration of marine organisms and suspended particles. Computational solutions will include autofocus routines, search learning algorithms, a robust database, and 3D velocity measurements. Tracking trajectories and automatic holographic reconstruction tools will allow us to acquire unique data both in laboratory observations and in natural and industrial environments. Such approach will provide information on a wide range of processes, from predator-prey interactions in dense particle suspensions to the detection of tiny organisms and particles in fast-flowing systems characterized by turbulent boundary layers such as in a ballast water discharge line on board commercial ships. The developed system will be architecturally modular to meet specific application demands.
Ecology, Applied Optics, Imaging, Holography, Digital Holography, Plankton, Particle Detection and Sensoring
During all the HARPIA project we will work on the fundamental property of the holography: an holographically stored image is the photographically or otherwise recorded interference pattern between a wave field scattered from the object and a coherent background named reference wave. It is usually recorded on a surface, but contains the information about the entire three-dimensional wave field. This information is coded in form of interference stripes, usually not visible for the human eye due to the high spatial frequencies. The reconstruction software for the holographic image is the decoder to access at the three dimensional nature of the image. We will implement a software interface for visualizing and processing the state of the art reconstructed images coming from the hardware setup at the Laboratory of Plankton Systems, Oceanographic Institute, University of São Paulo (LAPS/IOUSP). Worldwide computational efforts are related to reduce both the time-machine for speeding up the processing (approaching as much as possible to the real-time visualization) and the percentage error in image recognition. It is challenging today to find the best algorithm to compensate the temporal gap between the capture process of the image and the numerical reconstruction.
Different strategies can be adopted in relation to the context where the scanning holography microscopy is used.
Months 1-3: set-up in-line holographic system installation and initial database acquisition/classification
Months 4-6: the first release of the software for the research investigation
Months 7-11: second release of the software - the autofocus
Months 12-17: third release of the software - the pattern recognition
Months 18-24: fourth release of the software - particle tracking

Solicitações

Laboratory of Plankton Systems (LAPS/IOUSP)
- Optical passive components (lenses, objectives, posts, holders, pinholes, spatial filters, filters)
- Optical active components (lasers, LEDS)
- Digital recording cameras
- Acquaria
- Microscope
Zooplankton, Phytoplakton
CEBIMar, Terminal Marítimo Almirante Barroso (TEBAR)
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- Digital phase microscopy - FlowCam
  • NEC_COLLEC
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