Parecer da Comissão Científica

Projeto do CEBIMar

Dados do solicitante

David Suggett

Natureza do projeto

Projeto de docente ou pesquisador
Projeto temático

Pesquisadores ou docentes associados

Philippe Tortell
Aurea Maria Ciotti
Maxim Gorbunov
Nina Schuback
Ilana Berman-Frank

Recursos

UNESCO
International Council for Science

Descrição do projeto

Active Chlorophyll fluorescence for autonomous measurements of global marine primary productivity
15-09-2018
13-09-2024
Marine primary productivity controls ocean food webs and biogeochemical cycles, exerting a strong influence on CO2 uptake from the atmosphere and global climate. Unprecedented anthropogenic pressure has created an urgent need to understand environmental controls on primary productivity. This, in turn, relies on consistent and coherent measurements across a range of spatial and temporal scales. Productivity estimates from conventional 14C-uptake experiments require discrete bottle sampling (and suffer potential experimental artefacts), while those from mixed layer dissolved gas measurements (O2, CO2 etc.) do not directly measure gross photosynthesis, and lack the temporal resolution needed to validate daily remote-sensing observations. Active chlorophyll a (Chla) fluorescence-based measurements can overcome these challenges. First introduced several decades ago, techniques such as Fast Repetition Rate fluorometry have significantly advanced our understanding of environmental controls on phytoplankton physiology and productivity. However, rapidly growing capacity to engineer and deploy sea-going fluorometers now poses a major time-sensitive challenge: Conceptual, operational and computational approaches to extract and interpret fluorescence parameters are rapidly diverging. While an increasing number of (often custom-built) sensors, protocols and processing algorithms is being produced, no standard best practices have been formally adopted by the research community. Rapidly growing data sets may thus become increasingly difficult (perhaps impossible) to reconcile, thereby limiting our capacity to integrate observations over large-scales. This SCOR working group will address this challenge by producing international standards for best-practices in the acquisition and interpretation of active Chl fluorescence data, while also creating a framework for a global synthesis of existing and future data.
marine phytoplankton, techniques, photo physiology, active fluorescence
We will conduct a SCOR-funded laboratory inter-calibration of the state-of-the-art instrumentation, covering the broadest range of commercial and custom hardware and software (FRRf, FRRf-type single pulse, FRRf-flash, LIFT; as well as mini-FIRe and PicoF). This exercise will examine inter-comparability among existing configurations, studying the effects of various sources of variability (hardware and software) on parameter retrieval. We will conduct this exercise at a relatively central location amongst the WG members (Vancuver) using a range of marine phytoplankton cultures (with different pigment complements, cell size, taxonomic group) grown under various experimental conditions, including light and nutrient availability. We will conduct field work at CEBIMar, to intercompare two instruments focussing on their spectral correction. We will develop a standard set of protocols, including hardware configurations, parameter selection, algorithm assumptions, data formatting, sample collection/treatment. All participants will contribute in their various expertise to a user guide and best-practices report. This work is expected to lead to a significant peer-reviewed publication. The group will also engage early in the program with existing relevant platforms to assist in communication and global adoption of established standards and best-practices. Notably, the “Ocean Best Practice repository”.
Year 1 (2019): Kick-off meeting; laboratory inter-comparison study
Year 2: Field evaluations of best-practice
Year 3: Begin ‘legacy phase’ through software and database development
Year 4: Remote sensing integration and public release

Due to the pandemics this original Working Plan and Time-line changed.
We have worked mainly online, and decided to include a final comparison exercise regarding the influence of the light fields in nature in Sepetember-October at CEBIMar/USP (Brazil).

Solicitações

Laboratorio Aquarela and preparation lab
Laboratorio Aquarela
Water samples, plankton
São Sebastião Channel.
yes, low wind and waves, moderate currents
Sim
  • Auxílio técnico para coleta de organismos ou observações de campo
  • Utilização de embarcação do CEBIMar
  • Setembro
  • Outubro
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