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Projeto do NP-BioMar

Dados do solicitante

José Roberto Machado Cunha da Silva

Natureza do projeto

Projeto de docente ou pesquisador
Outra - Convênio de pesquisa internacional

Pesquisadores ou docentes associados

Elisa Bergami
Ilaria Corsi
João Carlos Shimada Borges
Luciana Machado Dzik
Andrews Krupinski Emerenciano

Recursos

Processo: 407904/2013-1 - Projeto: ISOLAMENTO E COMPARAÇÃO DOS EQUINOCROMOS DOS OURIÇOS TROPICAL Lytechinus variegatus (Lamark, 1816) E ANTÁRTICO Sterechinus neumayeri (Meissner, 1900) SUAS POTENCIAIS APLICAÇÕES FARMACOLÓGICAS E TERAPÉUTICAS
CNPq

Descrição do projeto

Efeito do acúmulo de nanopartículas plásticas em ouriços-do-mar Lytechinus variegatus e Echinometra lucunter
08-03-2016
30-12-2019
A recent study estimates that trillions of plastics are floating all over the oceans (Eriksen et al., 2014), representing one of the most important threats for marine ecosystems together with global warming and ocean acidification. Although Antarctica has been historically seen as pristine region, even this remote area could be reached by plastic debris crossing the Antarctic Polar Front. Macroplastics 1 cm have been surveyed by sight in the Southern Ocean since 1980s (Ivar do Sul et al., 2011) and despite the low densities, their effective presence and high persistence (Barnes et al., 2009) mightpose a serious concern for these fragile marine ecosystems. Up to date, no information is available on micro 5mm and nanoplastics 100nm in Antarctica resulting from weathering and fragmentation processes of macrodebris despite the presence of microplastics around Australia and the remote Southern Ocean Island of South Georgia have been recently reported (Reisser et al., 2013). Smaller plastic pieces including nanoplastics can be easily ingested and accumulated by small invertebrates, leading to trophic transfer (Boerger et al., 2010; Cole et al., 2013) and then reaching top-predators (e.g. fishes and penguins). Sunspension feeders as well as bottom grazer, which occupy a large portion of Antarctica biodiversity (Brey et al., 1995), are the primary biological targets of micro and nanoplastics, being exposed both to polymeric beads in suspension and to the fraction in sediments (Matranga and Corsi, 2012; Manzo et al., 2013; Della Torre et al., 2014). Considering that Antarctic marine ecosystems are characterised by an extremely short trophic web, small perturbations at the lower trophic levels can heavily affect the whole ecosystems.
microplástico, ouriço do mar, Lytechinus, Echinometra.
Based on the current experience of the UNISI-DSFTA RU1 on nanoparticles impact on marine biota including nanoplastics and in collaboration with the Brazilian partners of UNISP who performed extensive studies on Antarctic invertebrates species and their responses to environmental disturbance, several in vivo exposure experiments will be performed jointly both during the Brazilian Antarctic expedition ( year 1) and on available facilities in Brazil (RU3) and Italy (RU1 and RU2)(year 2). Micro and nanoplastics uptake, bioaccumulation and toxicity (i.e. mechanisms) will be thus investigated by using both molecular and cellular tools (details in section 1.7) using Antarctic filter feeders (krill) and botton grazer.
Parallel experiments (year 2) will be performed using tropical and Mediterranean species of the same trophic level as for instance the sea urchin Lytechinus variegates or Echinometra lucunter at the CEBIMar facility, the Marine Biology Center of the University of São Paulo UNISP (Brazil) (RU3) and with Paracentrotus lividus at the aquarium facility of the UNISI DSFTA (Italy) (RU2).
Mesocosms in vivo experiments will be also performed at facilities available at IAMC CNR (Italy) (RU2) where a trophic web will be used (including bacteria, zooplankton, sea urchin and fish) meant to represent a more realistic exposure scenarios in which fate, bioaccumulation and toxicity of model micro and nanoplastics will be investigated.
Março - abril de 2016, coleta do material e realização dos experimentos.
Maio, processamento no campus Butantan da USP.
O projeto tem término previsto para dezembro de 2018, mas por enquanto, só há previsão de uma estadia no CEBIMar em março/abril de 2016. Se houver necessidade de outras estadias, serão nos mesmos moldes da solicitada para março/abril de 2016 e avisaremos com antecedência.

Solicitações

Uma pequena bancada para manipulação dos animais e o uso de um microscópio de fluorescência.
Microscópio de fluorescência com contraste de fase.
Lytechinus variegatus e Echinometra lucunter e alguns filtradores bentônicos
Canal de São Sebastião.
{"0":"EC_ANY"}
Espaço no biotério para manutenção dos ouriços experimentais com água corrente.
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